Eukaryotic initiation factor 5A: the molecular form of the hypusine-containing protein from human erythrocytes

S I Chung; M H Park; J E Folk; M S Lewis

doi:10.1016/0167-4838(91)90490-q

Eukaryotic initiation factor 5A: the molecular form of the hypusine-containing protein from human erythrocytes

Biochim Biophys Acta. 1991 Feb 15;1076(3):448-51. doi: 10.1016/0167-4838(91)90490-q.

Authors

S I Chung¹, M H Park, J E Folk, M S Lewis

Affiliation

¹ National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892.

PMID: 1900436
DOI: 10.1016/0167-4838(91)90490-q

Abstract

Eukaryotic initiation factor 5A (eIF-5A, formerly known as eIF-4D) purified from human erythrocytes has been found to have a monomeric molecular weight between 17,500 and 18,000. In this study, using exclusion chromatography and analytical ultracentrifugation, we demonstrate that eIF-5A normally exists as a dimer in solution and appears to be capable of undergoing reversible association to form higher polymers.

MeSH terms

Chromatography, Gel
Electrophoresis, Polyacrylamide Gel
Eukaryotic Translation Initiation Factor 5A
Humans
Lysine / analogs & derivatives*
Lysine / analysis
Molecular Weight
Peptide Initiation Factors / blood*
Peptide Initiation Factors / isolation & purification
Protein Denaturation
RNA-Binding Proteins*

Substances

Peptide Initiation Factors
RNA-Binding Proteins
hypusine
Lysine