The C-terminal domains of adenovirus serotype 5 protein IX assemble into an antiparallel structure on the facets of the capsid

J Virol. 2009 Jan;83(2):1135-9. doi: 10.1128/JVI.01808-08. Epub 2008 Nov 12.


Adenovirus serotype 5 protein IX (pIX) has two domains connected by a flexible linker. Three N-terminal domains form triskelions on the capsid facets that cement hexons together, and the C-terminal domains of four monomers form complexes toward the facet periphery. Here we present a cryoelectron microscopy structure of recombinant adenovirus with a peptide tag added to the C terminus of pIX. The structure, made up by several C termini of pIX, is longer at both ends than the wild-type protein, and Fabs directed against the tag bind to both ends of the oligomer, demonstrating that the pIX C termini associate in an antiparallel manner.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoviridae / chemistry
  • Adenoviridae / physiology*
  • Adenoviridae / ultrastructure*
  • Antibodies, Viral / metabolism
  • Capsid Proteins / chemistry
  • Capsid Proteins / metabolism*
  • Capsid Proteins / ultrastructure*
  • Cryoelectron Microscopy
  • Humans
  • Models, Molecular
  • Protein Binding
  • Protein Structure, Quaternary


  • Antibodies, Viral
  • Capsid Proteins
  • hexon capsid protein, Adenovirus