Qualitative Human Immunodeficiency Virus RNA Analysis of Dried Blood Spots for Diagnosis of Infections in Infants

J Clin Microbiol. 2009 Jan;47(1):220-2. doi: 10.1128/JCM.01521-08. Epub 2008 Nov 12.

Abstract

The Gen-Probe Aptima human immunodeficiency virus type 1 (HIV-1) RNA assay was adapted for the diagnosis of HIV infection in infants by using dried blood spots. The assay was 99% sensitive (128/129) and 100% specific (162/162). This may prove useful in resource-limited settings, since it precludes the need for a phlebotomist and maintenance of a cold chain.

Publication types

  • Evaluation Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Blood / virology*
  • Desiccation
  • HIV Infections / diagnosis*
  • HIV-1 / genetics*
  • Humans
  • Infant
  • RNA, Viral / blood*
  • RNA, Viral / genetics*
  • Sensitivity and Specificity
  • Specimen Handling / methods*

Substances

  • RNA, Viral