Identification of the HERV-K gag antigen in prostate cancer by SEREX using autologous patient serum and its immunogenicity

Cancer Immun. 2008 Nov 13;8:15.

Abstract

The prostate cancer HERV-K gag-related NGO-Pr-54 antigen was identified by SEREX analysis using autologous patient serum. NGO-Pr-54 mRNA was observed to be faintly expressed in normal prostate and strongly expressed in a variety of cancers, including ovarian cancer (5/8), prostate cancer (6/9), and leukemia (5/14). A phage plaque assay showed that a strong reaction was constantly observed with clone ZH042 in which the 5' end of NGO-Pr-54 is deleted, suggesting that it contained the sequence coding for the protein product. A TI-35 mAb was produced using a recombinant protein (438 aa) deduced from the sequence of ZH042. Transfection of clone ZH042 into 293T cells resulted in the production of an approximately 50-kDa molecule visualized by Western blotting. Natural production of the molecule was confirmed in a SK-MEL-23 melanoma cell line. An indirect immunofluorescence assay showed that NGO-Pr-54 protein was expressed on the cell surface as well as in the cytoplasm. Cell surface expression was confirmed by flow cytometry using the TI-35 mAb. The antibody response against NGO-Pr-54 was observed in patients with bladder (5.1%), liver (4.1%), lung (3.4%), ovarian (5.6%), and prostate (4.2%) cancer, as well as with malignant melanoma (13.2%).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged, 80 and over
  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal
  • Antigens, Neoplasm / genetics*
  • Antigens, Neoplasm / immunology
  • COS Cells
  • Chlorocebus aethiops
  • Cloning, Molecular
  • Endogenous Retroviruses
  • Female
  • Fluorescent Antibody Technique, Indirect
  • Gene Expression Regulation, Neoplastic
  • Gene Products, gag*
  • Humans
  • Immune Sera / analysis
  • Leukemia / genetics
  • Leukemia / immunology
  • Male
  • Melanoma / genetics
  • Melanoma / immunology
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Neoplasm Proteins / genetics*
  • Ovarian Neoplasms / genetics
  • Ovarian Neoplasms / immunology
  • Prostate / immunology
  • Prostate / metabolism
  • Prostatic Neoplasms / genetics*
  • Prostatic Neoplasms / immunology
  • Recombinant Proteins / genetics
  • Transfection

Substances

  • Antibodies, Monoclonal
  • Antigens, Neoplasm
  • Gene Products, gag
  • Immune Sera
  • NGO-Pr-54 protein, human
  • Neoplasm Proteins
  • Recombinant Proteins