Barley transformation using Agrobacterium-mediated techniques

Methods Mol Biol. 2009;478:137-47. doi: 10.1007/978-1-59745-379-0_9.

Abstract

Methods for the transformation of barley using Agrobacterium-mediated techniques have been available for the past 10 years. Agrobacterium offers a number of advantages over biolistic-mediated techniques in terms of efficiency and the quality of the transformed plants produced. This chapter describes a simple system for the transformation of barley based on the infection of immature embryos with Agrobacterium tumefaciens followed by the selection of transgenic tissue on media containing the antibiotic hygromycin. The method can lead to the production of large numbers of fertile, independent transgenic lines. It is therefore ideal for studies of gene function in a cereal crop system.

MeSH terms

  • Agrobacterium tumefaciens / metabolism*
  • Coculture Techniques
  • Culture Media
  • Gene Transfer Techniques*
  • Hordeum / cytology
  • Hordeum / genetics*
  • Hordeum / growth & development
  • Hordeum / microbiology*
  • Plants, Genetically Modified
  • Seeds / cytology
  • Seeds / genetics
  • Seeds / growth & development
  • Seeds / microbiology
  • Tissue Culture Techniques
  • Transformation, Genetic*

Substances

  • Culture Media