Inhibiting XIAP expression by RNAi to inhibit proliferation and enhance radiosensitivity in laryngeal cancer cell line

Auris Nasus Larynx. 2009 Jun;36(3):332-9. doi: 10.1016/j.anl.2008.08.006. Epub 2008 Nov 13.

Abstract

Objectives: X-linked inhibitor of apoptosis protein (XIAP) is a novel member of the inhibitors of apoptosis (IAPs) family. The overexpression of XIAP is asscociated with radioresistance of human malignancies. The purpose of the present study was to investigate the effect of shRNA-targeted XIAP on the proliferation, apoptosis and radiosensitivity of human laryngeal carcinoma cells (Hep-2).

Methods: A siRNA expression vector (pSilencer4.1-XIAPshRNA) was constructed and stably transfected into human laryngeal carcinoma cells (Hep-2). The downregulation of XIAP expression was evaluated by RT-PCR and Western blot analyses. Then, we investigated the effect of XIAP-shRNA on the proliferation, cell cycle changes and apoptosis in vitro of Hep-2 cells. Finally, the radiosensitivity of Hep-2 cells was investigated by clonogenic cell survival assay.

Results: We established stably transfected cell line (Hep-2/XIAPshRNA) in which the expression of XIAP gene was downregulated. The cell viability of Hep-2/XIAP-RNA cells was obviously decreased compared with that of untransfected Hep-2 cells. Morever, XIAP-shRNA induced cell arrest in the G(0)/G(1) phase of cell cycle by flow cytometry analysis. Results of TUNEL assay indicated that Hep-2 cells stably transfected pSilencer4.1-XIAP-shRNA showed obvious apoptosis characters. Furthermore, the downregulation of XIAP expression could lead to significant radiosensitivity enhancement in laryngeal carcinoma cells.

Conclusions: RNAi-mediated downregulation of XIAP expression can inhibit proliferation, induce apoptosis and diminish the radioresistance of laryngeal carcinoma cells, so combined therapy with XIAP inhibition and radiation may be a potential strategy for the treatment of laryngeal carcinoma.

MeSH terms

  • Apoptosis / physiology
  • Blotting, Western
  • Carcinoma / genetics*
  • Carcinoma / therapy*
  • Cell Cycle
  • Cell Line, Tumor
  • Cell Proliferation
  • DNA, Complementary / genetics
  • Down-Regulation
  • Flow Cytometry
  • Humans
  • In Vitro Techniques
  • Laryngeal Neoplasms / genetics*
  • Laryngeal Neoplasms / therapy*
  • RNA Interference*
  • Radiation Tolerance*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transfection
  • X-Linked Inhibitor of Apoptosis Protein / genetics*

Substances

  • DNA, Complementary
  • X-Linked Inhibitor of Apoptosis Protein
  • XIAP protein, human