Genetic or pharmaceutical blockade of p110delta phosphoinositide 3-kinase enhances IgE production

J Allergy Clin Immunol. 2008 Oct;122(4):811-819.e2. doi: 10.1016/j.jaci.2008.08.008.

Abstract

Background: Recent studies indicate that pharmaceutical blockade of phosphoinositide 3-kinase (PI3K) signaling enzymes might be effective in reducing allergic airway inflammation. Signals generated by the p110delta PI3K isoform play critical roles in signaling through antigen and cytokine receptors and were shown to be required for induction of type 2, but not type 1, cytokine responses.

Objective: We sought to determine the effect of genetic or pharmaceutical inactivation of p110delta PI3K on induction of IgE responses.

Methods: We determined the effect of p110delta inactivation on induction of systemic IgE responses and on the ability of purified B lymphocytes to undergo IgE isotype switch in vitro. IgG and IgE germline transcription, postswitch transcription, protein expression, and secretion were measured, as well as cell division and expression of activation-induced cytidine deaminase, an enzyme required for isotype switch.

Results: Paradoxically, inactivation of p110delta PI3K led to markedly increased IgE responses, despite reduced production of other antibody isotypes. This result was seen by using genetic inactivation of p110delta inhibition with IC87114 compound or blockade with the broad-spectrum PI3K inhibitors PIK-90 and PI-103. Significant increases in IgG1/IgE double-positive cells were observed, indicating that inactivation of PI3K leads to uncontrolled sequential switching from IgG1 to IgE. Disruption of p110delta signaling results in increased germline transcription at the epsilon locus and increased activation-induced cytidine deaminase expression, suggesting deregulation at the level of the isotype switch process.

Conclusion: Blockade of PI3K signaling leads to markedly enhanced B-cell switch to IgE and increased IgE levels in vivo, despite reduced type 2 cytokine production.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • B-Lymphocytes / enzymology*
  • Cells, Cultured
  • Cytidine Deaminase / biosynthesis
  • Cytidine Deaminase / genetics
  • Enzyme Activation / drug effects
  • Enzyme Activation / genetics
  • Enzyme Inhibitors / pharmacology*
  • Furans / pharmacology*
  • Gene Expression Regulation, Enzymologic / drug effects*
  • Gene Expression Regulation, Enzymologic / genetics
  • Immunoglobulin Class Switching / drug effects
  • Immunoglobulin Class Switching / genetics
  • Immunoglobulin E / biosynthesis*
  • Immunoglobulin E / genetics
  • Mice
  • Mice, Transgenic
  • Phosphatidylinositol 3-Kinases / genetics
  • Phosphatidylinositol 3-Kinases / metabolism
  • Phosphoinositide-3 Kinase Inhibitors*
  • Pyridines / pharmacology*
  • Pyrimidines / pharmacology*
  • Signal Transduction / drug effects
  • Signal Transduction / genetics

Substances

  • Enzyme Inhibitors
  • Furans
  • PI103
  • Phosphoinositide-3 Kinase Inhibitors
  • Pyridines
  • Pyrimidines
  • Immunoglobulin E
  • Pik3cd protein, mouse
  • Cytidine Deaminase