Abstract
Interleukin (IL)-6 stimulates lipolysis in human and rodents adipocytes. However, the mechanism regulating this process is little known. In this study, we demonstrated that IL-6 increased lipolysis in differentiated porcine adipocytes by activation of extracellular signal-related kinase (ERK), which was inhibited by specific ERK inhibitor PD98059. IL-6 treatment did not elevate intracellular cAMP and specific PKA inhibitor H89 did not affect IL-6-induced lipolysis, which suggested that protein kinase A (PKA) pathway was not involved in IL-6-induced lipolysis. Also, the expressions of perilipin A and PPARgamma2 were significantly reduced in response to IL-6 treatment, but the expressions of peroxisome proliferators-activated receptor gamma coactivator-1 alpha (PGC-1alpha), carnitinepalmitoyl-transferase-1 (CPT-1), and uncoupling protein 2 (UCP2) were significantly elevated. In conclusion, these results suggested that chronic high dose of IL-6 directly stimulated lipolysis in porcine adipocytes through activation of ERK, subsequently repressing perilipin A and promoting PGC-1alpha expression.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adipocytes / cytology
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Adipocytes / drug effects
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Adipocytes / metabolism*
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Animals
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Carrier Proteins
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Cell Differentiation
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Cells, Cultured
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Cyclic AMP-Dependent Protein Kinases / antagonists & inhibitors
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Cyclic AMP-Dependent Protein Kinases / metabolism
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Cytokine Receptor gp130 / genetics
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Cytokine Receptor gp130 / metabolism
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Extracellular Signal-Regulated MAP Kinases / metabolism
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Fatty Acids, Nonesterified / metabolism
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Female
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Flavonoids / pharmacology
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Gene Expression / physiology
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Interleukin-6 / metabolism*
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Interleukin-6 / pharmacology
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Isoquinolines / pharmacology
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Lipolysis / drug effects
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Lipolysis / physiology*
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MAP Kinase Signaling System / drug effects
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MAP Kinase Signaling System / physiology*
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Male
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Oxidation-Reduction
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PPAR gamma / genetics
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PPAR gamma / metabolism
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Perilipin-1
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Phosphoproteins / genetics
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Phosphoproteins / metabolism
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Protein Kinase Inhibitors / pharmacology
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Sulfonamides / pharmacology
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Sus scrofa
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Transcription Factors / genetics
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Transcription Factors / metabolism
Substances
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Carrier Proteins
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Fatty Acids, Nonesterified
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Flavonoids
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Interleukin-6
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Isoquinolines
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PPAR gamma
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Perilipin-1
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Phosphoproteins
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Protein Kinase Inhibitors
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Sulfonamides
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Transcription Factors
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peroxisome-proliferator-activated receptor-gamma coactivator-1
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Cytokine Receptor gp130
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Cyclic AMP-Dependent Protein Kinases
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Extracellular Signal-Regulated MAP Kinases
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N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide
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2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one