Immersion oil for high-resolution live-cell imaging at 37 degrees C: optical and physical characteristics

J Microsc. 2008 Nov;232(2):353-61. doi: 10.1111/j.1365-2818.2008.02106.x.


The use of normal immersion oil, developed for 23 degrees C, at 37 degrees C greatly compromises both axial resolution and signal intensity. We developed and characterized an immersion oil for optimal performance in live-cell imaging at 37 degrees C. We quantify the improvements in resolution and intensity obtained when using the new oil instead of its standard 23 degrees C counterparts.

MeSH terms

  • Animals
  • Cells, Cultured
  • Kidney / cytology
  • Mice
  • Microscopy, Confocal / methods*
  • Microscopy, Fluorescence / methods*
  • Oils*
  • Temperature*


  • Oils