Transcriptome analysis and identification of regulators for long-term plasticity in Aplysia kurodai

Proc Natl Acad Sci U S A. 2008 Nov 25;105(47):18602-7. doi: 10.1073/pnas.0808893105. Epub 2008 Nov 18.

Abstract

The marine mollusk Aplysia is a useful model organism for studying the cellular bases of behavior and plasticity. However, molecular studies of Aplysia have been limited by the lack of genomic information. Recently, a large scale characterization of neuronal transcripts was performed in A. californica. Here, we report the analysis of a parallel set of neuronal transcripts from a closely related species A. kurodai found in the northwestern Pacific. We collected 4,859 nonredundant sequences from the nervous system tissue of A. kurodai. By performing microarray and real-time PCR analyses, we found that ApC/EBP, matrilin, antistasin, and eIF3e clones were significantly up-regulated and a BAT1 homologous clone was significantly down-regulated by 5-HT treatment. Among these, we further demonstrated that the Ap-eIF3e plays a key role in 5-HT-induced long-term facilitation (LTF) as a positive regulator.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aplysia / metabolism
  • Aplysia / physiology*
  • Base Sequence
  • DNA Primers
  • Expressed Sequence Tags
  • Long-Term Potentiation / drug effects
  • Neuronal Plasticity*
  • Polymerase Chain Reaction
  • RNA, Messenger / genetics*
  • Serotonin / pharmacology

Substances

  • DNA Primers
  • RNA, Messenger
  • Serotonin