Expression and functional analyses of circadian genes in mouse oocytes and preimplantation embryos: Cry1 is involved in the meiotic process independently of circadian clock regulation

Biol Reprod. 2009 Mar;80(3):473-83. doi: 10.1095/biolreprod.108.069542. Epub 2008 Nov 19.

Abstract

In mammals, circadian genes, Clock, Arntl (also known as Bmal1), Cry1, Cry2, Per1, Per2, and Per3, are rhythmically transcribed every 24 h in almost all organs and tissues to tick the circadian clock. However, their expression and function in oocytes and preimplantation embryos have not been investigated. In this study we found that the circadian clock may stop in mouse oocytes and preimplantation embryos. Real-time PCR analysis revealed the presence of transcripts of these genes in both oocytes and preimplantation embryos; however, their amounts did not oscillate every 24 h in one- to four-cell and blastocyst-stage embryos. Moreover, immunofluorescence analyses revealed that CLOCK, ARNTL, and CRY1 were localized similarly in the nuclei of germinal vesicle (GV) oocytes and one-cell- to four-cell-stage embryos. Because CRY1 is known to interact with the CLOCK-ARNTL complex to suppress transcription-promoting activity of the complex for genes such as Wee1, Cry2, Per1, Per2, and Per3 in cells having the ticking circadian clock, we hypothesized that if the circadian clock functions in GV oocytes and one-cell- to four-cell-stage embryos, CLOCK, ARNTL, and CRY1 might suppress the transcription of these genes in GV oocytes and one-cell- to 4-cell-stage embryos as well. As a result, knockdown of CRY1 in GV oocytes by RNA interference did not affect the transcription levels of Wee1, Cry2, Per1, Per2, and Per3, but it reduced maturation ability. Thus, it seems that circadian genes are not involved in circadian clock regulation in mouse oocytes and preimplantation embryos but are involved in physiologies, such as meiosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ARNTL Transcription Factors
  • Animals
  • Basic Helix-Loop-Helix Transcription Factors / metabolism*
  • Blastocyst / cytology
  • Blastocyst / metabolism*
  • CLOCK Proteins
  • Cell Cycle Proteins / metabolism
  • Cell Nucleus / metabolism
  • Cells, Cultured
  • Circadian Rhythm / physiology*
  • Coculture Techniques
  • Cryptochromes
  • Female
  • Flavoproteins / metabolism*
  • Intracellular Signaling Peptides and Proteins / metabolism
  • Male
  • Meiosis / physiology*
  • Mice
  • Mice, Inbred ICR
  • Nuclear Proteins / metabolism
  • Oocytes / cytology
  • Oocytes / metabolism*
  • Period Circadian Proteins
  • Protein-Tyrosine Kinases / metabolism
  • RNA, Small Interfering / pharmacology
  • Trans-Activators / metabolism*
  • Transcription Factors / metabolism

Substances

  • ARNTL Transcription Factors
  • Arntl protein, mouse
  • Basic Helix-Loop-Helix Transcription Factors
  • Cell Cycle Proteins
  • Cry1 protein, mouse
  • Cry2 protein, mouse
  • Cryptochromes
  • Flavoproteins
  • Intracellular Signaling Peptides and Proteins
  • Nuclear Proteins
  • Per1 protein, mouse
  • Per2 protein, mouse
  • Per3 protein, mouse
  • Period Circadian Proteins
  • RNA, Small Interfering
  • Trans-Activators
  • Transcription Factors
  • CLOCK Proteins
  • Clock protein, mouse
  • Protein-Tyrosine Kinases
  • Wee1 protein, mouse