Label-free in vivo optical imaging of microvasculature and oxygenation level

J Biomed Opt. Jul-Aug 2008;13(4):040503. doi: 10.1117/1.2968260.


The ability to perform high-resolution imaging of microvasculature and its oxygenation is very important in studying early tumor development. Toward this goal, we improved upon our excited state absorption (ESA)-based imaging technique to allow us to not only image hemoglobin directly but also differentiate between oxy- and deoxyhemoglobin in tissue. We demonstrate the separation of arterioles from venules in a live nude mouse ear using our imaging technique.

Publication types

  • Letter

MeSH terms

  • Animals
  • Blood Flow Velocity / physiology*
  • Ear / blood supply
  • Ear / physiology
  • Mice
  • Mice, Nude
  • Microscopy, Confocal / methods*
  • Microscopy, Fluorescence, Multiphoton / methods*
  • Microvessels / cytology*
  • Microvessels / physiology*
  • Oximetry / methods*
  • Oxygen / metabolism*
  • Oxygen Consumption / physiology


  • Oxygen