Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. Sep-Oct 2008;13(5):054041.
doi: 10.1117/1.2981830.

Second Harmonic Microscopy to Quantify Renal Interstitial Fibrosis and Arterial Remodeling

Affiliations
Free article

Second Harmonic Microscopy to Quantify Renal Interstitial Fibrosis and Arterial Remodeling

Mathias Strupler et al. J Biomed Opt. .
Free article

Abstract

Interstitial fibrosis is a powerful pejorative predictor of progression of nephropathies in a variety of chronic renal diseases. It is characterized by the depletion of kidney cells and their replacement by extracellular matrix, in particular, type-I fibrillar collagen, a protein scarce in normal interstitium. However, assessment of fibrosis remains a challenge in research and clinical pathology. We develop a novel methodology based on second harmonic generation (SHG) microscopy, and we image collagen fibers in human and mouse unstained kidneys. We take into account the variability in renal shape, and we develop automated image processing for quantitative scoring of thick murine tissues. This approach allows quantitative 3-D imaging of interstitial fibrosis and arterial remodeling with high accuracy. Moreover, SHG microscopy helps to raise pathophysiological questions. First, imaging of a large volume within a mouse kidney shows that progression of fibrosis is a heterogeneous process throughout the different renal compartments. Second, SHG from fibrillar collagens does not overlap with the glomerular tuft, despite patent clinical and experimental glomerulosclerosis. Since glomerulosclerosis involves SHG-silent nonfibrillar collagens, our work supports pathophysiological differences between interstitial fibrosis and glomerulosclerosis, a clearly nonfibrotic process.

Similar articles

See all similar articles

Cited by 22 articles

See all "Cited by" articles

Publication types

Feedback