Chlorogenic acids from green coffee extract are highly bioavailable in humans

J Nutr. 2008 Dec;138(12):2309-15. doi: 10.3945/jn.108.095554.


Chlorogenic acids (CGA) are cinnamic acid derivatives with biological effects mostly related to their antioxidant and antiinflammatory activities. Caffeoylquinic acids (CQA) and dicaffeoylquinic acids (diCQA) are the main CGA found in nature. Because green coffee is a major source of CGA, it has been used for production of nutraceuticals. However, data on the bioavailability of CGA from green coffee in humans are inexistent. The present study evaluated the pharmacokinetic profile and apparent bioavailability of CGA in plasma and urine of 10 healthy adults for 8 h after the consumption of a decaffeinated green coffee extract containing 170 mg of CGA. Three CQA, 3 diCQA, and caffeic, ferulic, isoferulic, and p-coumaric acids were identified in plasma by HPLC-Diode Array Detector-MS after treatment. Over 30% (33.1 +/- 23.1%) of the ingested cinnamic acid moieties were recovered in plasma, including metabolites, with peak levels from 0.5 to 8 h after treatment. CGA and metabolites identified in urine after treatment were 4-CQA, 5-CQA, and sinapic, p-hydroxybenzoic, gallic, vanillic, dihydrocaffeic, caffeic, ferulic, isoferulic, and p-coumaric acids, totaling 5.5 +/- 10.6% urinary recovery of the ingested cinnamic and quinic acid moiteties. This study shows that the major CGA compounds present in green coffee are highly absorbed and metabolized in humans.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Biological Availability
  • Caffeic Acids / pharmacokinetics
  • Caffeine / isolation & purification
  • Chlorogenic Acid / administration & dosage
  • Chlorogenic Acid / blood
  • Chlorogenic Acid / pharmacokinetics*
  • Chlorogenic Acid / urine
  • Cinnamates / pharmacokinetics
  • Coffee / chemistry*
  • Coffee / metabolism*
  • Coumaric Acids / pharmacokinetics
  • Female
  • Humans
  • Male
  • Middle Aged
  • Propionates
  • Quinic Acid / analogs & derivatives
  • Quinic Acid / pharmacokinetics
  • Young Adult


  • Caffeic Acids
  • Cinnamates
  • Coffee
  • Coumaric Acids
  • Propionates
  • caffeoylquinic acid
  • Quinic Acid
  • Chlorogenic Acid
  • Caffeine
  • ferulic acid
  • p-coumaric acid
  • caffeic acid
  • isoferulic acid