A validated gas chromatographic-electron impact ionization mass spectrometric method for methamphetamine, methylenedioxymethamphetamine (MDMA), and metabolites in mouse plasma and brain

J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Dec 15;876(2):266-76. doi: 10.1016/j.jchromb.2008.11.001. Epub 2008 Nov 7.


A method was developed and fully validated for simultaneous quantification of methamphetamine (MAMP), amphetamine, hydroxy-methamphetamine, methylenedioxymethamphetamine (MDMA, ecstasy), methylenedioxyamphetamine, 3-hydroxy-4-methoxy-methamphetamine, and 3-hydroxy-4-methoxy-amphetamine in 100 microL mouse plasma and 7.5mg brain. Solid phase extraction and gas chromatography-electron impact ionization mass spectrometry in selected-ion monitoring mode achieved plasma linear ranges of 10-20 to 20,000 ng/mL and 0.1-0.2 to 200 ng/mg in brain. Recoveries were greater than 91%, bias 92.3-110.4%, and imprecision less than 5.3% coefficient of variation. This method was used for measuring MAMP and MDMA and metabolites in plasma and brain during mouse neurotoxicity studies.

Publication types

  • Evaluation Study
  • Research Support, N.I.H., Intramural

MeSH terms

  • Animals
  • Brain Chemistry
  • Gas Chromatography-Mass Spectrometry / methods*
  • Humans
  • Hydrolysis
  • Male
  • Methamphetamine / analysis*
  • Methamphetamine / blood*
  • Methamphetamine / metabolism
  • Mice
  • N-Methyl-3,4-methylenedioxyamphetamine / analysis*
  • N-Methyl-3,4-methylenedioxyamphetamine / blood*
  • N-Methyl-3,4-methylenedioxyamphetamine / metabolism
  • Sensitivity and Specificity
  • Spectrometry, Mass, Electrospray Ionization / methods


  • Methamphetamine
  • N-Methyl-3,4-methylenedioxyamphetamine