High-level transgene expression in neurons by lentivirus with Tet-Off system

Neurosci Res. 2009 Feb;63(2):149-54. doi: 10.1016/j.neures.2008.10.010. Epub 2008 Nov 6.


We developed novel lentiviral vectors by using "Tet-Off system" and succeeded in achieving high-level and neuron-specific gene transduction in vivo. One week after viral injection into the rat neostriatum, the GFP expression was almost completely neuron-specific and about 40 times higher than the expression of a conventional lentiviral vector. High transcriptional activity and neuronal specificity were sustained for up to 8 weeks. Furthermore, neuronal processes of the infected neurons were efficiently visualized by adding a plasma membrane-targeting signal to GFP. These results suggest that the present method is valuable for strong gene transduction and clear visualization of neurons in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analysis of Variance
  • Animals
  • Functional Laterality
  • Gene Expression Regulation
  • Genetic Vectors / administration & dosage*
  • Genetic Vectors / genetics
  • Green Fluorescent Proteins / genetics
  • Humans
  • Lentivirus / genetics*
  • Male
  • Neostriatum / cytology
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism
  • Neurons / cytology
  • Neurons / metabolism*
  • Promoter Regions, Genetic
  • Rats
  • Rats, Wistar
  • Time Factors
  • Transduction, Genetic / methods*


  • Nerve Tissue Proteins
  • Green Fluorescent Proteins