Human embryonic stem cells reveal recurrent genomic instability at 20q11.21

Nat Biotechnol. 2008 Dec;26(12):1364-6. doi: 10.1038/nbt.1509. Epub 2008 Nov 23.


By analyzing five human embryonic stem (hES) cell lines over long-term culture, we identified a recurrent genomic instability in the human genome. An amplification of 2.5-4.6 Mb at 20q11.21, encompassing approximately 23 genes in common, was detected in four cell lines of different origins. This amplification, which has been associated with oncogenic transformation, may provide a selective advantage to hES cells in culture.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Chromosomes, Human, Pair 20 / genetics*
  • Comparative Genomic Hybridization
  • Embryonic Stem Cells / cytology*
  • Gene Amplification
  • Genomic Instability*
  • Humans
  • In Situ Hybridization, Fluorescence
  • Molecular Sequence Data
  • Oligonucleotide Array Sequence Analysis
  • Polymorphism, Single Nucleotide
  • Recurrence

Associated data

  • GEO/GSE13565