B cells play a crucial role as antigen-presenting cells and collaborate with inflammatory cytokines in glucose-6-phosphate isomerase-induced arthritis

Clin Exp Immunol. 2009 Feb;155(2):285-94. doi: 10.1111/j.1365-2249.2008.03816.x. Epub 2008 Nov 20.


Anti-glucose-6-phosphate isomerase (GPI) antibodies from K/BxN mice directly induce arthritis; however, the transfer of these antibodies from mice with GPI-induced arthritis does not induce arthritis. CD4(+) T cells play an important role in the induction and effector phase in this model; however, the roles of B cells and immunoglobulins (Igs) have not been elucidated. We investigated the roles of B cells and Igs in GPI-induced arthritis by using adoptive transfer system into SCID mice. Transfer of splenocytes of male DBA/1 mice immunized with GPI into SCID mice induced arthritis on day 6 in the latter, in association with the production of anti-GPI antibodies. Co-localization of C3 and IgG on the articular surface was identified in arthritic SCID mice. Inoculation of IgG (or anti-GPI antibodies) and CD19(+)-depleted splenocytes from arthritic DBA/1 mice induced arthritis in SCID mice, but not CD19(+)-depleted or CD4(+)-depleted splenocytes from DBA/1 mice. In vitro analysis of cytokine production by splenocytes from DBA/1 arthritic mice demonstrated production of large amounts of tumour necrosis factor (TNF)-alpha and interleukin (IL)-6 in an antigen-specific manner (P < 0.01), and production was dominated by CD19(+)-depleted than CD4(+)-depleted splenocytes (P < 0.05). Addition of IgG from DBA/1 arthritic mice to the culture enhanced TNF-alpha but not IL-6 production, and this effect was blocked by anti-Fcgamma receptor antibody. In vivo analysis of neutralization with TNF-alpha protected arthritis completely in SCID mice. Our results highlight the important role of B cells in GPI-induced arthritis as autoantibody producers, and these autoantibodies can trigger joint inflammation in orchestration with inflammatory cytokines, especially TNF-alpha.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigen Presentation / immunology*
  • Antigens, CD19 / analysis
  • Arthritis, Experimental / chemically induced
  • Arthritis, Experimental / immunology*
  • Arthritis, Experimental / pathology
  • B-Lymphocytes / immunology*
  • CD11b Antigen / analysis
  • CD4-Positive T-Lymphocytes / immunology
  • Cell Communication / immunology
  • Cells, Cultured
  • Coculture Techniques
  • Cytokines / immunology*
  • Glucose-6-Phosphate Isomerase
  • Interleukin-6 / biosynthesis
  • Male
  • Mice
  • Mice, Inbred DBA
  • Mice, SCID
  • Spleen / immunology
  • Spleen / transplantation
  • Tumor Necrosis Factor-alpha / biosynthesis


  • Antigens, CD19
  • CD11b Antigen
  • Cytokines
  • Interleukin-6
  • Tumor Necrosis Factor-alpha
  • Glucose-6-Phosphate Isomerase