Purification of recombinant Gi alpha and Go alpha proteins from Escherichia coli

Methods Enzymol. 1991;195:202-15. doi: 10.1016/0076-6879(91)95167-i.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Chromatography, Affinity / methods
  • Chromatography, High Pressure Liquid / methods
  • Chromatography, Ion Exchange / methods
  • Cloning, Molecular / methods
  • Electrophoresis, Polyacrylamide Gel / methods
  • Escherichia coli / genetics
  • GTP-Binding Proteins / genetics
  • GTP-Binding Proteins / isolation & purification*
  • GTP-Binding Proteins / metabolism
  • Guanosine 5'-O-(3-Thiotriphosphate) / metabolism
  • Indicators and Reagents
  • Macromolecular Substances
  • Molecular Sequence Data
  • Molecular Weight
  • NAD / metabolism
  • Oligonucleotide Probes
  • Rats
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism
  • Restriction Mapping
  • Virulence Factors, Bordetella / metabolism

Substances

  • Indicators and Reagents
  • Macromolecular Substances
  • Oligonucleotide Probes
  • Recombinant Proteins
  • Virulence Factors, Bordetella
  • NAD
  • Guanosine 5'-O-(3-Thiotriphosphate)
  • GTP-Binding Proteins