Abstract
Reliable methods for the detection of cytomegalovirus (CMV) strain-specific serological responses are lacking. We describe a simple and reliable enzyme-linked immunosorbent assay method developed to detect antibodies against the polymorphic epitopes within the two envelope glycoproteins of CMV, glycoproteins H and B. This assay is useful for the detection of serologic responses to CMV strains and the identification of CMV reinfections.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Amino Acid Sequence
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Antibodies, Viral / blood*
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Cytomegalovirus / immunology*
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Cytomegalovirus Infections / immunology*
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Enzyme-Linked Immunosorbent Assay / methods
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Epitopes, B-Lymphocyte / immunology
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Humans
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Molecular Sequence Data
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Viral Envelope Proteins / immunology
Substances
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Antibodies, Viral
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Epitopes, B-Lymphocyte
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Viral Envelope Proteins
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glycoprotein B, Simplexvirus
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glycoprotein H, Cytomegalovirus