High-throughput Protein Production--Lessons From Scaling Up From 10 to 288 Recombinant Proteins Per Week

Biotechnol J. 2009 Jan;4(1):51-7. doi: 10.1002/biot.200800183.

Abstract

The demand for high-throughput recombinant protein production has markedly increased with the increased activity in the field of proteomics. Within the Human Protein Atlas project recombinantly produced human protein fragments are used for antibody production. Here we describe how the protein expression and purification protocol has been optimized in the project to allow for handling of nearly 300 different proteins per week. The number of manual handling steps has been significantly reduced (from 18 to 9) and the protein purification has been completely automated.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Automation*
  • Biotechnology / instrumentation*
  • Chromatography, Affinity
  • Electrophoresis, Polyacrylamide Gel
  • Escherichia coli / genetics
  • Peptides / genetics
  • Peptides / metabolism
  • Proteomics / instrumentation*
  • Recombinant Proteins / genetics*
  • Recombinant Proteins / metabolism
  • Spectrophotometry

Substances

  • Peptides
  • Recombinant Proteins