Dihydrofolate reductase from a resistant subline of the L1210 lymphoma. Purification by affinity chromatography and ultraviolet difference spectrophotometric and circular dichroic studies

Biochemistry. 1977 Jul 12;16(14):3073-9. doi: 10.1021/bi00633a005.
No abstract available

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Chromatography, Affinity
  • Circular Dichroism
  • Drug Resistance
  • Leukemia L1210 / enzymology*
  • Methotrexate
  • Molecular Weight
  • NADP
  • Spectrophotometry, Ultraviolet
  • Tetrahydrofolate Dehydrogenase* / isolation & purification
  • Tryptophan / analysis

Substances

  • NADP
  • Tryptophan
  • Tetrahydrofolate Dehydrogenase
  • Methotrexate