T-cell responses against tuberculin and sensitin in children with tuberculosis and non-tuberculosis mycobacterial lymphadenopathy

Clin Microbiol Infect. 2008 Nov;14(11):1079-83. doi: 10.1111/j.1469-0691.2008.02084.x.

Abstract

Multi-colour flow cytometry was applied to determine T-cell-specific interferon-gamma, interleukin-2 and tumour necrosis factor-alpha expression in children with tuberculosis and non-tuberculosis mycobacterial lymphadenopathy (NTM-L). In vitro stimulation of peripheral blood mononuclear cells with purified protein derivative from Mycobacterium tuberculosis (tuberculin) and M. avium (sensitin) revealed differential recognition of tuberculin and sensitin in both study groups. Ratios of tuberculin-specific and sensitin-specific T-cell proportions in individual patients discriminated between children with tuberculosis or NTM-L. These findings have the potential to improve the differential diagnosis of mycobacterial infections.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens / immunology*
  • Cells, Cultured
  • Child
  • Child, Preschool
  • Female
  • Flow Cytometry
  • Humans
  • Infant
  • Interferon-gamma / biosynthesis
  • Interleukin-2 / biosynthesis
  • Leukocytes, Mononuclear / immunology
  • Lymphatic Diseases / immunology*
  • Male
  • Mycobacterium Infections / immunology*
  • T-Lymphocytes / immunology*
  • Tuberculin / immunology*
  • Tuberculosis, Lymph Node / immunology*
  • Tumor Necrosis Factor-alpha / biosynthesis

Substances

  • Antigens
  • Interleukin-2
  • Tuberculin
  • Tumor Necrosis Factor-alpha
  • sensitin
  • Interferon-gamma