A new spectrophotometric method for the determination of tianeptine in tablets using ion-pair reagents

Chem Pharm Bull (Tokyo). 2008 Dec;56(12):1635-8. doi: 10.1248/cpb.56.1635.


A new rapid and sensitive procedure assay is proposed for the spectrophotometric determination of tianeptine. The developed method involves formation of colored chloroform extractable ion-pair complexes of tianeptine with bromophenol blue (BPB), bromocresol green (BCG), bromothymol blue (BTB) and methyl orange (MO) in acidic medium. Beer's law is obeyed in the concentration ranges 3.0-12.0, 4.0-16.0, 4.0-14.0 and 2.0-10.0 microg ml(-1) with BPB, BCG, BTB and MO, respectively. The detection limit of tianeptine was found to be 1.8 microg ml(-1) for BPB, 2.0 for BCG, 2.0 microg ml(-1) for BTB and 1.0 microg ml(-1) for MO. Validation of the method was performed in terms of linearity, limit of detection (LOD), quantification (LOQ), accuracy and precision. Common excipients used as additives in pharmaceutical preparations do not interfere in the proposed method. The proposed method has been applied to determination of the examined drugs in pharmaceutical formulations and the results demonstrated that the method is equally accurate, precise, and reproducible as the official method. The t-test showed no significant difference at 95% confidence level.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antidepressive Agents, Tricyclic / analysis*
  • Chemistry, Pharmaceutical
  • Hydrogen-Ion Concentration
  • Indicators and Reagents
  • Reference Standards
  • Reproducibility of Results
  • Solvents
  • Spectrophotometry, Ultraviolet
  • Tablets
  • Temperature
  • Thiazepines / analysis*


  • Antidepressive Agents, Tricyclic
  • Indicators and Reagents
  • Solvents
  • Tablets
  • Thiazepines
  • tianeptine