Cloning and nucleotide sequence of the anaerobically regulated pepT gene of Salmonella typhimurium

J Bacteriol. 1991 Jun;173(11):3554-8. doi: 10.1128/jb.173.11.3554-3558.1991.


The anaerobically regulated pepT gene of Salmonella typhimurium has been cloned in pBR328. Strains carrying the pepT plasmid, pJG17, overproduce peptidase T by approximately 70-fold. The nucleotide sequence of a 2.5-kb region including pepT has been determined. The sequence codes for a protein of 44,855 Da, consistent with a molecular weight of approximately 46,000 for peptidase T (as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and gel filtration). The N-terminal amino acid sequence of peptidase T purified from a pJG17-containing strain matches that predicted by the nucleotide sequence. A plasmid carrying an anaerobically regulated pepT::lacZ transcriptional fusion contains only 165 bp 5' to the start of translation. This region contains a sequence highly homologous to that identified in Escherichia coli as the site of action of the FNR protein, a positive regulator of anaerobic gene expression. A region of the deduced amino acid sequence of peptidase T is similar to segments of Pseudomonas carboxypeptidase G2, the E. coli peptidase encoded by the iap gene, and E. coli peptidase D.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Aminopeptidases / analysis
  • Aminopeptidases / genetics*
  • Aminopeptidases / isolation & purification
  • Base Sequence
  • Cloning, Molecular
  • Genes, Bacterial*
  • Molecular Sequence Data
  • Restriction Mapping
  • Salmonella typhimurium / genetics*
  • Sequence Homology, Nucleic Acid
  • beta-Galactosidase / biosynthesis


  • beta-Galactosidase
  • Aminopeptidases
  • peptidase T

Associated data

  • GENBANK/M62725
  • GENBANK/M62937
  • GENBANK/M62938
  • GENBANK/M62939
  • GENBANK/M62940
  • GENBANK/M62941
  • GENBANK/M62942
  • GENBANK/M62943
  • GENBANK/M62944
  • GENBANK/M62945