Different solid-phase extraction (SPE) cartridges for the isolation of most of the phenolic compounds present in wines in low concentration were assayed and compared with the C18 SPE sorbent, which is the most common sorbent used for the isolation of these compounds. The use of C18 cartridges has several disadvantages; therefore, a SPE method using copolymer cartridges was developed, optimized, and validated. The method proposed seems to be a good alternative to replace C18 cartridges for the isolation of wine phenolic compounds. The advantages of the proposed SPE method with the HLB cartridge are that interferences can be eliminated with water without losing the compounds of great interest; the method has very good repeatability, reproducibility, and high percentages of recovery; it has a higher sensitivity and loading capacity than silica-based C18 cartridges due to the larger surface area of this type of sorbent, and the method is not adversely affected by drying, being more reproducible than C18 cartridge.