The murine rac1 gene: cDNA cloning, tissue distribution and regulated expression of rac1 mRNA by disassembly of actin microfilaments

Oncogene. 1991 May;6(5):863-6.

Abstract

A 886 bp cDNA encoding the murine rac1 protein has been isolated. The abundance of rac1 mRNA was determined in fourteen tissues from both mouse and pig. The mRNA 5' non-coding sequence is very rich in G + C, and has the potential to form several stable secondary structures. In addition, this region contains a putative open reading frame of 57 amino acids. Disruption of the actin microfilament network by cytochalasin B in LLC-PK1 cells results in down regulation of rac1 mRNA. In agreement with the proposed role of rac1 in exocytosis, these results could explain the inhibitory effect of cytochalasin B on secretory processes.

MeSH terms

  • Actin Cytoskeleton / physiology*
  • Actin Cytoskeleton / ultrastructure
  • Actins / physiology*
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Cloning, Molecular
  • Colchicine / pharmacology
  • Cytochalasin B / pharmacology
  • Cytoskeleton / drug effects
  • DNA / genetics*
  • DNA / isolation & purification
  • GTP-Binding Proteins / genetics*
  • Gene Expression Regulation* / drug effects
  • Gene Library
  • Mice
  • Molecular Sequence Data
  • Nocodazole / pharmacology
  • Nucleic Acid Conformation
  • Oligonucleotide Probes
  • Organ Specificity
  • RNA, Messenger / analysis
  • RNA, Messenger / drug effects
  • RNA, Messenger / genetics
  • Swine
  • rac GTP-Binding Proteins

Substances

  • Actins
  • Oligonucleotide Probes
  • RNA, Messenger
  • Cytochalasin B
  • DNA
  • GTP-Binding Proteins
  • rac GTP-Binding Proteins
  • Nocodazole
  • Colchicine