Proteomic analysis of HepaRG cells: a novel cell line that supports hepatitis B virus infection

J Proteome Res. 2009 Jan;8(1):118-22. doi: 10.1021/pr800562j.


The first proteomic characterization of the HepaRG cell line, the only cell line that is susceptible to hepatitis B virus (HBV) infection and supports a complete virus life cycle, is reported. Differential analysis of naive and HBV-infected HepaRG cells by two-dimensional gel electrophoresis revealed 19 differentially regulated features, 7 increasing and 12 decreasing with HBV infection. The proteins identified in these features were involved in various cellular pathways including apoptosis, DNA/RNA processing, and hepatocellular impairment. Similar expression changes in a number of the identified proteins have already been reported for other virus systems. Identification of these expression changes is a validation of the proteomics approach and contributes to an understanding of host cellular response to HBV infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis
  • Cell Line
  • DNA / chemistry
  • Electrophoresis, Gel, Two-Dimensional
  • Hepatitis B / metabolism*
  • Hepatitis B virus / metabolism*
  • Hepatocytes / metabolism
  • Hepatocytes / virology*
  • Humans
  • Image Processing, Computer-Assisted
  • Mass Spectrometry
  • Phenotype
  • Proteome
  • Proteomics / methods*
  • RNA / chemistry


  • Proteome
  • RNA
  • DNA