Grifola frondosa (maitake mushroom) water extract inhibits vascular endothelial growth factor-induced angiogenesis through inhibition of reactive oxygen species and extracellular signal-regulated kinase phosphorylation

J Med Food. 2008 Dec;11(4):643-51. doi: 10.1089/jmf.2007.0629.


Grifola frondosa, a large edible mushroom also known as maitake, has been used as a health food for centuries in China and Japan. In the present study, we examined anti-angiogenic activity of a water extract of the fruiting body of G. frondosa (GFW). An in vivo angiogenesis assay using chick chorioallantoic membrane revealed that GFW (1-100 microg/mL) dose-dependently inhibited vascular endothelial growth factor (VEGF)-induced angiogenesis. In addition, GFW inhibited VEGF-induced proliferation, chemotactic migration, and capillary-like tube formation of human umbilical vein endothelial cells (HUVECs) in a concentration-dependent manner. Upon stimulation by VEGF, HUVECs rapidly increased reactive oxygen species production, which was significantly blocked by the treatment with GFW. Moreover, phosphorylation of extracellular signal-regulated kinase 1/2, a downstream signaling molecule following VEGF receptor activation, was also inhibited by GFW. The results indicate that GFW effectively inhibit angiogenesis by blocking VEGF signaling and suggest that G. frondosa fruiting body may be a valuable medicinal food for treatment of angiogenesis-associated human diseases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiogenesis Inhibitors / pharmacology*
  • Angiogenesis Inhibitors / therapeutic use
  • Animals
  • Antioxidants / pharmacology
  • Cell Culture Techniques
  • Chick Embryo
  • Dose-Response Relationship, Drug
  • Endothelial Cells
  • Extracellular Signal-Regulated MAP Kinases / drug effects*
  • Extracellular Signal-Regulated MAP Kinases / metabolism
  • Fruiting Bodies, Fungal
  • Grifola*
  • Humans
  • Neovascularization, Pathologic / drug therapy*
  • Phosphorylation / drug effects
  • Phytotherapy*
  • Plant Extracts / pharmacology*
  • Plant Extracts / therapeutic use
  • Reactive Oxygen Species / antagonists & inhibitors*
  • Signal Transduction
  • Umbilical Veins
  • Vascular Endothelial Growth Factor A / metabolism*


  • Angiogenesis Inhibitors
  • Antioxidants
  • Plant Extracts
  • Reactive Oxygen Species
  • Vascular Endothelial Growth Factor A
  • Extracellular Signal-Regulated MAP Kinases