Identification and physiological evaluation of the components from citrus fruits as potential drugs for anti-corpulence and anticancer

Bioorg Med Chem. 2009 Jan 1;17(1):25-8. doi: 10.1016/j.bmc.2008.11.039. Epub 2008 Nov 21.


On the basis of monitoring the prevention of accumulation of lipid droplets in mouse 3T3-L1 preadipocyte cells and inhibition of the proliferation of human colon cancer HT-29 cells, effective anti-corpulence and anticancer compounds were isolated from the peel of Citrus fruits. These bioactive components were identified as polymethoxyflavones and coumarin derivatives by spectroscopic analyses. 5-Hydroxy-6,7,8,3',4'-pentamethoxyflavone had the greatest anti-corpulence effects and 3,5,6,7,8,3',5'-heptamethoxyflavone had the greatest anticancer effects. Furthermore, distributions of those bioactive components in the peel of 10 species of Citrus fruits were demonstrated by HPLC analyses.

MeSH terms

  • 3T3-L1 Cells
  • Adipocytes / drug effects
  • Animals
  • Anti-Obesity Agents / isolation & purification*
  • Anti-Obesity Agents / pharmacology
  • Antineoplastic Agents / isolation & purification*
  • Antineoplastic Agents / pharmacology
  • Cell Line, Tumor
  • Citrus / chemistry*
  • Coumarins / isolation & purification*
  • Coumarins / pharmacology
  • Drug Evaluation, Preclinical
  • Flavones / isolation & purification*
  • Flavones / pharmacology
  • Humans
  • Mice


  • Anti-Obesity Agents
  • Antineoplastic Agents
  • Coumarins
  • Flavones
  • coumarin