Validation of plasma clearance of 51Cr-EDTA in adult renal transplant recipients: comparison with inulin renal clearance

Transpl Int. 2009 Mar;22(3):323-31. doi: 10.1111/j.1432-2277.2008.00799.x. Epub 2008 Dec 3.


Plasma clearance of (51)Cr-EDTA ((51)Cr-EDTA-Cl) is an alternative method to evaluate glomerular filtration rate (GFR). This study aimed to investigate the concordance between (51)Cr-EDTA-Cl and renal inulin clearance (In-Cl) in renal transplant recipients as well to determine the repeatability of (51)Cr-EDTA-Cl in kidney donors. Forty four kidney recipients and 22 kidney donors were enrolled. Simultaneous measurements of (51)Cr-EDTA-Cl and In-Cl were performed. A single dose of 3.7MBq of (51)Cr-EDTA was injected and the plasma disappearance curve was created by taking blood samples at 2, 4, 6 and 8 h after injection. Bland and Altman statistical approach was used to quantify the agreement between In-Cl and (51)Cr-EDTA-Cl and to determine the better concordance between all possibilities of measure for the (51)Cr-EDTA-Cl. The mean of In-Cl was 44.5 +/- 17.9 ml/min/1.73 m(2). There was a positive correlation between In-Cl and all possible measurements of (51)Cr-EDTA-Cl. (51)Cr-EDTA-Cl with two samples taken at 4 and 8 h or at 4 and 6 h presenting the narrow limits of agreement and a difference (bias) of 2.8 and 2.7 ml/min, respectively. Two plasma sampling for (51)Cr-EDTA-Cl was a reliable method to measure GFR compared with In-Cl and comprises a suitable method to be used in kidney transplanted patients.

Publication types

  • Clinical Trial
  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Adult
  • Anticoagulants* / pharmacokinetics
  • Chromium Radioisotopes
  • Edetic Acid* / pharmacokinetics
  • Female
  • Glomerular Filtration Rate
  • Humans
  • Hypoglycemic Agents / pharmacokinetics
  • Insulin / pharmacokinetics
  • Kidney Function Tests / methods*
  • Kidney Function Tests / standards*
  • Kidney Transplantation*
  • Male
  • Middle Aged
  • Models, Biological


  • Anticoagulants
  • Chromium Radioisotopes
  • Hypoglycemic Agents
  • Insulin
  • Edetic Acid