Collagens of the chicken eggshell membranes

Connect Tissue Res. 1991;26(1-2):37-45. doi: 10.3109/03008209109152162.

Abstract

An immunohistochemical analysis of the eggshell membranes shows the occurrence of type X collagen while type I collagen was not detected by using an appropriate monoclonal antibody with untreated shell membranes. A positive immuno-reaction for type I collagen was obtained after digestion of the shell membranes with pepsin. These observations indicate the possibility that type I collagen epitope was masked by type X collagen and that type X collagen may serve as an inhibitory boundary for biomineralization.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetates / pharmacology
  • Acetic Acid
  • Animals
  • Antibodies, Monoclonal
  • Chick Embryo
  • Collagen / analysis*
  • Egg Shell / chemistry*
  • Fluorescent Antibody Technique
  • Glycoside Hydrolases*
  • Hyaluronoglucosaminidase / pharmacology
  • Membranes / chemistry
  • Minerals / metabolism
  • Pepsin A / pharmacology
  • beta-Galactosidase / pharmacology

Substances

  • Acetates
  • Antibodies, Monoclonal
  • Minerals
  • Collagen
  • Glycoside Hydrolases
  • keratan-sulfate endo-1,4-beta-galactosidase
  • beta-Galactosidase
  • Hyaluronoglucosaminidase
  • Pepsin A
  • Acetic Acid