Abstract
An immunohistochemical analysis of the eggshell membranes shows the occurrence of type X collagen while type I collagen was not detected by using an appropriate monoclonal antibody with untreated shell membranes. A positive immuno-reaction for type I collagen was obtained after digestion of the shell membranes with pepsin. These observations indicate the possibility that type I collagen epitope was masked by type X collagen and that type X collagen may serve as an inhibitory boundary for biomineralization.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acetates / pharmacology
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Acetic Acid
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Animals
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Antibodies, Monoclonal
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Chick Embryo
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Collagen / analysis*
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Egg Shell / chemistry*
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Fluorescent Antibody Technique
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Glycoside Hydrolases*
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Hyaluronoglucosaminidase / pharmacology
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Membranes / chemistry
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Minerals / metabolism
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Pepsin A / pharmacology
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beta-Galactosidase / pharmacology
Substances
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Acetates
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Antibodies, Monoclonal
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Minerals
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Collagen
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Glycoside Hydrolases
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keratan-sulfate endo-1,4-beta-galactosidase
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beta-Galactosidase
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Hyaluronoglucosaminidase
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Pepsin A
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Acetic Acid