Mitochondrial dysfunction leads to reduced chronological lifespan and increased apoptosis in yeast

FEBS Lett. 2009 Jan 5;583(1):113-7. doi: 10.1016/j.febslet.2008.11.028. Epub 2008 Dec 4.

Abstract

We previously isolated a Saccharomyces cerevisiae mutant (HsTnII), which displays 40% reduced chronological lifespan as compared to the wild type (WT). In this study, we found HsTnII cultures to be characterized by fragmented and dysfunctional mitochondria, and by increased initiation of apoptosis during chronological aging as compared to WT. Expression of genes encoding subunits of mitochondrial electron transport chain and ATP synthase is significantly downregulated in HsTnII, and as a consequence, HsTnII is not able to respire ethanol. All these data confirm the importance of functional mitochondria and respiration in determining yeast chronological lifespan and apoptosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis* / genetics
  • DNA Transposable Elements / genetics
  • Gene Expression
  • Hydrogen Peroxide / pharmacology
  • Mitochondria / physiology*
  • Mitochondria / ultrastructure
  • Protein-Serine-Threonine Kinases / genetics
  • Protein-Serine-Threonine Kinases / metabolism
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / physiology*
  • Saccharomyces cerevisiae / ultrastructure
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism

Substances

  • DNA Transposable Elements
  • Saccharomyces cerevisiae Proteins
  • Hydrogen Peroxide
  • Protein-Serine-Threonine Kinases
  • SCH9 protein, S cerevisiae