Inhibitory effect of dimethylthiourea on rat urinary bladder inflammation produced by 6-hydroxydopamine application

Auton Neurosci. 2009 Jan 28;145(1-2):44-9. doi: 10.1016/j.autneu.2008.11.003. Epub 2008 Dec 6.


The present study was to investigate 6-hydroxydopamine (6-OHDA)-induced inflammatory response and underlying mechanisms in the urinary bladder in anesthetized male rats of Long-Evans strain. The magnitude of inflammation was evaluated by morphometric analysis of the relative number of leaky blood vessels expressed by the area density of India ink-labeled blood vessels in whole mount specimens. Light and scanning electron microscopies were employed to study the changes in histologic structure and endothelial ultrastructure of bladder wall. Local injection of 6-OHDA to lumen of urinary bladder induced a dose-dependent increase in plasma leakage. Following application of vehicle, 5 mg/kg 6-OHDA, and 10 mg/kg 6-OHDA, area densities of India ink-labeled leaky vessels were 5.65+/-3.72% (N=6), 22.63+/-5.12% (N=6), and 35.02+/-11.25% (N=6), respectively. Inflammatory response was completely abolished by pretreatment alone with dimethylthiourea (DMTU), a hydroxyl radical scavenger, and was also attenuated by pretreatment with L-732,138, a NK1 receptor antagonist. 6-OHDA caused edema formation and venular endothelial gap formation in bladder tissue. It is concluded that 6-OHDA induced inflammation in the rat urinary bladder, the response of which was dose-dependently increased and free radicals and tachykinins were involved in the inflammatory process.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Capillary Leak Syndrome / chemically induced
  • Capillary Leak Syndrome / metabolism
  • Capillary Leak Syndrome / prevention & control
  • Capillary Permeability / drug effects
  • Capillary Permeability / physiology
  • Cystitis / chemically induced*
  • Cystitis / metabolism
  • Cystitis / prevention & control*
  • Dose-Response Relationship, Drug
  • Inflammation / chemically induced
  • Inflammation / metabolism
  • Inflammation / prevention & control
  • Male
  • Oxidopamine / antagonists & inhibitors
  • Oxidopamine / toxicity*
  • Rats
  • Rats, Long-Evans
  • Thiourea / analogs & derivatives*
  • Thiourea / therapeutic use
  • Urinary Bladder / drug effects*
  • Urinary Bladder / metabolism
  • Urinary Bladder / pathology*


  • Oxidopamine
  • 1,3-dimethylthiourea
  • Thiourea