Enhanced secretion of biologically active murine interleukin-12 by Lactococcus lactis

Appl Environ Microbiol. 2009 Feb;75(3):869-71. doi: 10.1128/AEM.01728-08. Epub 2008 Dec 5.

Abstract

The novel signal peptide SLPmod was used for the secretion of murine interleukin-12 (mIL-12) by Lactococcus lactis. A >4-fold increase in secretion was observed when SLPmod was used instead of the Usp45-derived secretion signal. Oral delivery of this cytokine using the autoinducible host L. lactis FI5876 utilizing SLPmod resulted in a significant increase in mIL-12 plasma levels in mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Interleukin-12 / genetics
  • Interleukin-12 / metabolism*
  • Lactococcus lactis / genetics
  • Lactococcus lactis / metabolism*
  • Mice
  • Plasma / chemistry
  • Protein Sorting Signals
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism*

Substances

  • Protein Sorting Signals
  • Recombinant Proteins
  • Interleukin-12