Increased coherence of circadian rhythms in mature fibroblast cultures

J Biol Rhythms. 2008 Dec;23(6):483-8. doi: 10.1177/0748730408326682.


Circadian transcriptional rhythms in confluent NIH 3T3 and PER2::LUCIFERASE fibroblast cultures become more coherent as they mature. This enhanced coherence correlated with increased culture density, while inhibition of the cell cycle increased coherence in immature cultures. Mature cultures may therefore express more coherent rhythms for longer because of their reduced rate of cell division. The authors conclude that mature fibroblast cultures will be of great utility for high-throughput pharmacological and mutational circadian screening.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aphidicolin / pharmacology
  • Cell Cycle Proteins / genetics
  • Cell Division / physiology
  • Circadian Rhythm / physiology*
  • Enzyme Inhibitors / pharmacology
  • Fibroblasts / physiology*
  • Luciferases / genetics
  • Mice
  • NIH 3T3 Cells
  • Nuclear Proteins / genetics
  • Nucleic Acid Synthesis Inhibitors
  • Period Circadian Proteins
  • Transcription Factors / genetics
  • Transfection


  • Cell Cycle Proteins
  • Enzyme Inhibitors
  • Nuclear Proteins
  • Nucleic Acid Synthesis Inhibitors
  • Per2 protein, mouse
  • Period Circadian Proteins
  • Transcription Factors
  • Aphidicolin
  • Luciferases