Anti-inflammatory effect of buckwheat sprouts in lipopolysaccharide-activated human colon cancer cells and mice

Biosci Biotechnol Biochem. 2008 Dec;72(12):3148-57. doi: 10.1271/bbb.80324. Epub 2008 Dec 7.


In conducting an in vitro screening of ethanol extracts from various natural foods using a human colon cancer cell line (CoLoTC cells), an extract of buckwheat sprouts (ExtBS) was found to express significant anti-inflammatory activity. The anti-inflammatory activity of ExtBS was confirmed by oral administration of lipopolysaccharide (LPS) to mice. Inflammatory cytokines (interleukin 6 and tumor necrosis factor alpha) were markedly up-regulated in the spleen and liver from LPS-administrated mice, and combinatory treatment with LPS and ExtBS decreased up-regulation of them in both cytokines. Both serum cytokine levels corresponded to their gene expressions in tissues, but no anti-inflammatry effect in mice was observed when ExtBS was treated intraperitoneally. ExtBS oral administration also showed protective activity as to hepatic injury induced by galactosamine/LPS treatment. Based on these data, we suggest that ExtBS contains anti-inflammatory compounds.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / administration & dosage
  • Anti-Inflammatory Agents / pharmacology*
  • Cell Line, Tumor
  • Cell Survival / drug effects
  • Colonic Neoplasms / pathology*
  • Fagopyrum / chemistry*
  • Flavonoids / pharmacology
  • Galactosamine / toxicity
  • Gene Expression Regulation, Neoplastic / drug effects
  • Humans
  • Inflammation / chemically induced
  • Inflammation / metabolism
  • Interleukin-8 / genetics
  • Lipopolysaccharides / administration & dosage
  • Lipopolysaccharides / toxicity*
  • Liver / drug effects
  • Liver / injuries
  • Mice
  • Oligonucleotide Array Sequence Analysis
  • Plant Extracts / administration & dosage
  • Plant Extracts / pharmacology*
  • Plant Shoots / chemistry*
  • Reference Standards
  • Up-Regulation / drug effects


  • Anti-Inflammatory Agents
  • Flavonoids
  • Interleukin-8
  • Lipopolysaccharides
  • Plant Extracts
  • Galactosamine