Petroleum ether extract of Cissus quadrangularis (LINN) stimulates the growth of fetal bone during intra uterine developmental period: a morphometric analysis

Clinics (Sao Paulo). 2008 Dec;63(6):815-20. doi: 10.1590/s1807-59322008000600018.


Objective: The aim of the present study was to analyze the effect Cissus quadrangularis plant petroleum ether extract on the development of long bones during the intra-uterine developmental stage in rats.

Methods: Pregnant rats (n=12) were randomly assigned into either a control group (n=6) or a Cissus quadrangularis treatment (n=6) group. Pregnant rats in the Cissus quadrangularis group were treated with Cissus quadrangularis petroleum ether extract at a dose of 500 mg/kg body weight from gestation day 9 until delivery. The animals in the control group received an equal volume of saline. Newborn pups were collected from both groups for alizarin red S - alcian blue staining to differentiate ossified and unossified cartilage. The ossified cartilage (bone) was morphometrically analyzed using Scion image software.

Results: Morphometric analysis revealed that the percentage of the total length of ossified cartilage (bone) in pups born to treated dams was significantly higher (P<0.001- -0.0001) than that of the control group.

Conclusion: The results of the present study suggest that maternal administration of Cissus quadrangularis petroleum ether extract during pregnancy can stimulate the development of fetal bone growth during the intra-uterine developmental period.

MeSH terms

  • Alkanes*
  • Animals
  • Bone Development / drug effects*
  • Cissus / chemistry*
  • Female
  • Fetal Development / drug effects*
  • Plant Extracts / pharmacology*
  • Pregnancy
  • Random Allocation
  • Rats
  • Rats, Wistar
  • Toxicity Tests, Acute


  • Alkanes
  • Plant Extracts
  • naphtha