Counting and determining the viability of cultured cells

J Vis Exp. 2008 Jun 23;(16):752. doi: 10.3791/752.


Determining the number of cells in culture is important in standardization of culture conditions and in performing accurate quantitation experiments. A hemacytometer is a thick glass slide with a central area designed as a counting chamber. Cell suspension is applied to a defined area and counted so cell density can be calculated.

Publication types

  • Video-Audio Media

MeSH terms

  • Animals
  • Cell Count / methods*
  • Cell Culture Techniques / methods*
  • Cell Survival
  • Humans
  • Trypan Blue / chemistry


  • Trypan Blue