Heat shock protein 70 on Neuro2a cells is a putative receptor for Japanese encephalitis virus

Virology. 2009 Mar 1;385(1):47-57. doi: 10.1016/j.virol.2008.10.025. Epub 2008 Dec 7.


Japanese encephalitis virus (JEV) envelope (E) protein has been shown to play a critical role in attachment to cells. However, the receptor interacting with envelope protein has not been conclusively identified. Using mouse neuroblastoma (Neuro2a) cells and purified JEV-E protein in 'Virus Overlay Protein Binding Assay' followed by MALDI-TOF analysis, we identified 'heat shock protein 70' (Hsp70) as a possible receptor for JEV. Indirect immunofluorescence and flow-cytometry analysis demonstrated localization of Hsp70 on Neuro2a cell surface. Co-immunoprecipitation followed by Western blot analysis reconfirmed the interaction between Hsp70 and JEV-E protein. Further, anti-Hsp70 polyclonal-antibodies were able to block JEV entry into Neuro2a cells. Additionally, using the bioinformatic tool - FTDOCK, docking between the proteins was performed. Amongst six interacting structural poses studied one pose involving RGD motif on JEV-E and leucine(539) on Hsp70 displayed stable interaction. These observations indicate that Hsp70 serves as putative receptor for JEV in Neuro2A cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Baculoviridae / growth & development
  • Baculoviridae / metabolism
  • Cell Line, Tumor
  • Encephalitis Virus, Japanese / immunology
  • Encephalitis Virus, Japanese / physiology*
  • HSP70 Heat-Shock Proteins / immunology
  • HSP70 Heat-Shock Proteins / metabolism*
  • Mice
  • Neurons
  • Protein Binding
  • Receptors, Virus / chemistry
  • Receptors, Virus / isolation & purification*
  • Receptors, Virus / metabolism
  • Viral Proteins / metabolism
  • Virus Attachment*
  • Virus Internalization


  • HSP70 Heat-Shock Proteins
  • Receptors, Virus
  • Viral Proteins