Effects of gel lubricant on cervical cytology

Acta Cytol. 2008 Nov-Dec;52(6):654-8. doi: 10.1159/000325617.


Objective: To determine whether the use of lubricating gel during vaginal speculum examination affected cytologic interpretation in the conventional Pap smear.

Study design: Two consecutive cervical smears were obtained from 1334 patients undergoing Pap smear screening. The first smear (uncontaminated) was obtained using the routine collection technique. The second smear (gel-contaminated) was taken after applying a 1- to 1.5-cm ribbon of lubricating gel onto the external cervical os. Adequacy of Pap smear and discordance in diagnosis between the paired smears were examined.

Results: The proportion of unsatisfactory smears was significantly higher in the gel-contaminated smears, 12.1% vs. 1.7% (p < 0.01). This difference was consistent across all reproductive groups. For patients who had smears satisfactory for cytologic evaluation, the discordance in cytologic diagnosis between the gel-contaminated and uncontaminated smears from the same patient was 0.3%.

Conclusion: Lubricating gel contamination of the cervix can adversely affect adequacy and cytologic diagnosis in the conventional Pap smear.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cervix Uteri / pathology*
  • Female
  • Gels
  • Humans
  • Lubrication*
  • Papanicolaou Test*
  • Surgical Instruments
  • Uterine Cervical Neoplasms / diagnosis*
  • Vaginal Smears / methods*


  • Gels