Quantification of tripdiolide in human whole blood by liquid chromatography coupled with atmospheric pressure chemical ionization tandem mass spectrometry

Micong Jin; Xiaokun Ouyang; Yiwen Yang; Qilong Ren

doi:10.1016/j.talanta.2006.11.030

Quantification of tripdiolide in human whole blood by liquid chromatography coupled with atmospheric pressure chemical ionization tandem mass spectrometry

Talanta. 2007 Apr 30;72(2):582-6. doi: 10.1016/j.talanta.2006.11.030. Epub 2006 Dec 18.

Authors

Micong Jin¹, Xiaokun Ouyang, Yiwen Yang, Qilong Ren

Affiliation

¹ Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, China; Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, China.

PMID: 19071658
DOI: 10.1016/j.talanta.2006.11.030

Abstract

A liquid chromatographic-tandem mass spectrometric (LC-MS/MS) assay was developed and validated to determine tripdiolide in human whole blood using dexamethasone acetate as an internal standard (I.S.). Liquid-liquid extraction with ethyl acetate was used to isolate them from the biological matrix. Detection was performed on a mass spectrometer coupled with a negative atmospheric pressure chemical ionization (APCI) in the multiple-reaction monitoring (MRM) mode. The calibration curve was linear (r(2)=0.9973) in the concentration range of 0.5-100.0ng/mL in human whole blood with a lower limit of quantification of 0.5ng/mL. Intra-day and inter-day relative standard deviations (R.S.D.s) were less than 7.0 and 10.1%, respectively. Extraction recoveries of tripdiolide ranged from 80.5 to 90.1%. This assay can be used to determine trace tripdiolide in human whole blood.