A Newly Identified Bocavirus Species in Human Stool

J Infect Dis. 2009 Jan 15;199(2):196-200. doi: 10.1086/595831.

Abstract

Viral metagenomic analysis was used to identify a previously uncharacterized parvovirus species, "HBoV2," whose closest phylogenetic relative is the human bocavirus (HBoV). HBoV2 has a genomic organization identical to that of HBoV but has only 78%, 67%, and 80% identity, respectively, with the latter's NS1, NP1, and VP1/VP2 proteins. The study used polymerase chain reaction to detect HBoV2 sequences in 5 of 98 stool samples from Pakistani children and in 3 of 699 stool samples from Edinburgh. Nearly-full-length genome sequencing revealed the presence of 3 HBoV2 genotypes and evidence of recombination between genotypes. Further studies are necessary to identify anatomical sites of HBoV2 replication and potential associations with clinical symptoms or disease.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Bocavirus / classification*
  • Bocavirus / genetics
  • Bocavirus / isolation & purification*
  • Child
  • Child, Preschool
  • Feces / virology*
  • Genotype
  • Humans
  • Infant
  • Pakistan / epidemiology
  • Parvoviridae Infections / epidemiology*
  • Parvoviridae Infections / virology*
  • Phylogeny
  • Polymerase Chain Reaction
  • Recombination, Genetic
  • Sequence Analysis, DNA
  • Species Specificity