Repair of DNA lesions associated with triplex-forming oligonucleotides

Mol Carcinog. 2009 Apr;48(4):389-99. doi: 10.1002/mc.20501.


Triplex-forming oligonucleotides (TFOs) are gene targeting tools that can bind in the major groove of duplex DNA in a sequence-specific manner. When bound to DNA, TFOs can inhibit gene expression, can position DNA-reactive agents to specific locations in the genome, or can induce targeted mutagenesis and recombination. There is evidence that third strand binding, alone or with an associated cross-link, is recognized and metabolized by DNA repair factors, particularly the nucleotide excision repair pathway. This review examines the evidence for DNA repair of triplex-associated lesions.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Animals
  • DNA / chemistry
  • DNA / genetics*
  • DNA / metabolism
  • DNA Damage
  • DNA Repair*
  • Humans
  • Nucleic Acid Conformation*
  • Oligonucleotides / chemistry
  • Oligonucleotides / genetics*
  • Oligonucleotides / metabolism


  • Oligonucleotides
  • triplex DNA
  • DNA