Synthesis of CDP-activated ribitol for teichoic acid precursors in Streptococcus pneumoniae

J Bacteriol. 2009 Feb;191(4):1200-10. doi: 10.1128/JB.01120-08. Epub 2008 Dec 12.


Streptococcus pneumoniae has unusually complex cell wall teichoic acid and lipoteichoic acid, both of which contain a ribitol phosphate moiety. The lic region of the pneumococcal genome contains genes for the uptake and activation of choline, the attachment of phosphorylcholine to teichoic acid precursors, and the transport of these precursors across the cytoplasmic membrane. The role of two other, so far uncharacterized, genes, spr1148 and spr1149, in the lic region was determined. TarJ (spr1148) encodes an NADPH-dependent alcohol dehydrogenase for the synthesis of ribitol 5-phosphate from ribulose 5-phosphate. TarI (spr1149) encodes a cytidylyl transferase for the synthesis of cytidine 5'-diphosphate (CDP)-ribitol from ribitol 5-phosphate and cytidine 5'-triphosphate. We also present the crystal structure of TarI with and without bound CDP, and the structures present a rationale for the substrate specificity of this key enzyme. No transformants were obtained with insertion plasmids designed to interrupt the tarIJ genes, indicating that their function could be essential for cell growth. CDP-activated ribitol is a precursor for the synthesis of pneumococcal teichoic acids and some of the capsular polysaccharides. Thus, all eight genes in the lic region have a role in teichoic acid synthesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Binding Sites
  • Cytidine Diphosphate / metabolism*
  • Gene Expression Regulation, Bacterial
  • Gene Silencing
  • Models, Molecular
  • Molecular Sequence Data
  • Protein Binding
  • Ribitol / metabolism*
  • Streptococcus pneumoniae / genetics
  • Streptococcus pneumoniae / metabolism*
  • Teichoic Acids / biosynthesis*


  • Bacterial Proteins
  • Teichoic Acids
  • Ribitol
  • Cytidine Diphosphate