Nucleotide and derived amino acid sequence of the cyanogenic beta-glucosidase (linamarase) from white clover (Trifolium repens L.)

Plant Mol Biol. 1991 Aug;17(2):209-19. doi: 10.1007/BF00039495.


The nucleotide sequence and derived amino acid sequence of two different beta-glucosidase cDNA clones were determined. One clone (TRE104) was identified as the cyanogenic beta-glucosidase by homology with the N-terminal and internal peptide amino acid sequence of the purified enzyme. The biological function of the other beta-glycosidase (TRE361) is not known. Co-segregation of genomic restriction fragments uniquely identified by each cDNA clone shows that these two genes are linked in the white clover genome. Both TRE104 and TRE361 fragments co-segregate with cyanogenic beta-glucosidase activity. Extensive homology was found between the white clover beta-glucosidase sequences and a group of prokaryote and mammalian beta-glycosidases. This group of sequences has no homology with a separate set of beta-glucosidase genes isolated from fungi and the thermophilic bacterium Clostridium thermocellum.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Blotting, Southern
  • Cloning, Molecular
  • DNA / genetics
  • Gene Expression
  • Molecular Sequence Data
  • Plants / enzymology*
  • Plants / genetics
  • RNA, Messenger / genetics
  • beta-Glucosidase / genetics*


  • RNA, Messenger
  • DNA
  • cyanogenic beta-glucosidase
  • beta-Glucosidase

Associated data

  • GENBANK/S46824
  • GENBANK/S46826
  • GENBANK/X55687
  • GENBANK/X55688
  • GENBANK/X55689
  • GENBANK/X55690
  • GENBANK/X56268
  • GENBANK/X56269
  • GENBANK/X56733
  • GENBANK/X56734