Infrared laser-mediated gene induction in targeted single cells in vivo

Nat Methods. 2009 Jan;6(1):79-81. doi: 10.1038/nmeth.1278. Epub 2008 Dec 14.


We developed infrared laser-evoked gene operator (IR-LEGO), a microscope system optimized for heating cells without photochemical damage. Infrared irradiation causes reproducible temperature shifts of the in vitro microenvironment in a power-dependent manner. When applied to living Caenorhabditis elegans, IR-LEGO induced heat shock-mediated expression of transgenes in targeted single cells in a more efficient and less deleterious manner than a 440-nm dye laser and elicited physiologically relevant phenotypic responses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Caenorhabditis elegans / cytology*
  • Caenorhabditis elegans / genetics
  • Caenorhabditis elegans / radiation effects*
  • Caenorhabditis elegans Proteins / genetics*
  • Caenorhabditis elegans Proteins / metabolism
  • Calibration
  • Escherichia coli / radiation effects
  • Gene Expression Regulation / radiation effects*
  • Lasers*
  • Microscopy, Confocal / instrumentation*
  • Microscopy, Confocal / methods*


  • Caenorhabditis elegans Proteins