Efficacy of a new combined larvicidal-adulticidal ultralow volume formulation against Aedes aegypti (Diptera: Culicidae), vector of dengue

Parasitol Res. 2009 Apr;104(5):1101-7. doi: 10.1007/s00436-008-1294-8. Epub 2008 Dec 10.


A new ultralow volume formulation (ULV) containing permethrin as an adulticidal active ingredient and the insect growth regulator (IGR) pyriproxyfen as a larvicide was developed and its efficacy evaluated in a field trial in Wanda, Misiones (Argentina). Two separate study areas were sprayed: one with a ULV formulation of permethrin 15% plus pyriproxyfen 3% and the other with permethrin 15% only. A third untreated area was kept as a control. Sentinel cages containing adult mosquitoes and jars containing Aedes aegypti larvae III/IV were placed in treated and control areas. The residual activity of the formulations was tested using 20 L water containers. The adulticide effect of permethrin 15% + pyriproxyfen 3% formulation was similar to the permethrin 15% formulation, however, the inhibition of adult emergence in the treatment with permethrin 15% never exceeded 20%, whereas the inhibition of adult emergence in the treatment with permethrin 15% plus pyriproxyfen 3% showed initial values of 96% maintaining this high level of inhibition up to 35 days after ULV spraying. Larval indexes (House and Breteau indexes) showed that a greater, long-lasting effect was obtained with the permethrin 15% plus pyriproxyfen 3% formulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aedes / drug effects*
  • Animals
  • Argentina
  • Disease Vectors*
  • Drug Interactions
  • Insecticides / administration & dosage
  • Insecticides / pharmacology*
  • Larva / drug effects
  • Permethrin / administration & dosage
  • Permethrin / pharmacology*
  • Pyridines / administration & dosage
  • Pyridines / pharmacology*


  • Insecticides
  • Pyridines
  • pyriproxyfen
  • Permethrin