Unequal representation of different unique genomic DNA sequences in the cell-free plasma DNA of individual donors

Clin Biochem. 2009 May;42(7-8):736-8. doi: 10.1016/j.clinbiochem.2008.11.006. Epub 2008 Dec 3.

Abstract

Objectives: To assess whether different genomic cell-free DNAs are equally abundant in the plasma of individual donors, and any relationship between DNA methylation and representation in plasma.

Design and methods: The concentrations of DNA in plasma were determined by real-time PCR.

Results: Different DNA sequences were not equally represented. The relative abundances were similar in different donors.

Conclusions: Different DNA sequences are not equally abundant in plasma, with no relationship between DNA methylation and abundance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA / blood*
  • DNA / chemistry*
  • Humans
  • Polymerase Chain Reaction
  • Sequence Analysis, DNA

Substances

  • DNA