Germline transformation of Caenorhabditis elegans by injection

Methods Mol Biol. 2009;518:123-33. doi: 10.1007/978-1-59745-202-1_10.


Microinjection is a commonly used technique for DNA transformation in Caenorhabditis elegans. It is a powerful tool that links genetic and molecular analysis to phenotypic analysis. In this chapter we shall provide an overview of microinjection for germline transformation in worms. Our discussion will emphasize C. elegans reproductive biology, applications and protocols for carrying out microinjection in order to successfully obtain transgenic worms.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Caenorhabditis elegans / genetics*
  • Caenorhabditis elegans / growth & development
  • Disorders of Sex Development
  • Germ Cells / metabolism*
  • Microinjections / methods*
  • Transformation, Genetic*